Penetration and Stability of Juvenile Hormone Analogues in Manduca sexta L. : Lepidoptera : Sphingidae
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چکیده
منابع مشابه
The Juvenile Hormone Binding Protein in the Hemolymph of Manduca sexta Johannson (Lepidoptera: Sphingidae).
C(18):juvenile hormone is quite soluble in water, yielding a monomeric solution greater than 10(-5) M. In vivo injection or addition of aqueous juvenile hormone to the hemolymph in vitro shows the complexation of juvenile hormone to a protein, as demonstrated by gel permeation chromatography and disc-gel electrophoresis. The protein has an apparent molecular weight of 3.4 x 10(4) and is present...
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Visual perception of depth change can be mediated monocularly by looming the apparent size increase of an approaching object. In Manduca sexta we recorded intracellularly from cells that detect both approach and retreat of an object. The cells compute looming in two fundamentally di!erent ways: class 1 neurons measure the change of perimeter/edge length of the object; class 2 neurons respond to...
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A new class of inhibitors of juvenile hormone epoxide hydrolase (JHEH) of Manduca sexta and further in vitro characterization of the enzyme are reported. The compounds are based on urea and amide pharmacophores that were previously demonstrated as effective inhibitors of mammalian soluble and microsomal epoxide hydrolases. The best inhibitors against JHEH activity so far within this class are N...
متن کاملPurification and characterization of the carrier protein for juvenile hormone from the hemolymph of the tobacco hornworm Manduca sexta Johannson (Lepidoptera: Sphingidae).
The larval hemolymph of the tobacco hornworm, Manduca sexta, contains a carrier protein that binds specifically and with high affinity the juvenile hormone, an important regulator of insect development. This protein serves to transport the hormone and to protect it from the action of degradative enzymes during early larval stages. Using hemolymph from the last larval stage, we have isolated a p...
متن کاملLigand blot analysis of juvenile hormone esterase binding proteins in Manduca sexta L.
Biotinylated recombinant juvenile hormone esterase (JHE) was used for ligand blotting of proteins from fat body tissue and pericardial athrocytes of Manduca sexta. Proteins were separated by SDS-polyacrylamide gel electrophoresis or by two-dimensional electrophoresis. Eight putative JHE binding proteins were detected in fat body tissue and in pericardial athrocytes of both M. sexta and Heliothi...
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ژورنال
عنوان ژورنال: Applied Entomology and Zoology
سال: 1987
ISSN: 0003-6862,1347-605X
DOI: 10.1303/aez.22.641